Plant Biotech / Plant Tissue Culture Notes

Plant Biotech / Plant Tissue Culture Notes

Plant Tissue Culture Techniques

Importance of Plant Tissue Culture, Importance and Historical View of PTC

Culture Media, Culture Media2, Sterilization Techniques, Totipotency, media1, media2

Types of Cultures, Single Cell Cultures, Meristem Culture, Micropropagation, Micropropagation Methods, Anther/ Pollen Culture, Application of Haploids in Plant Breeding, Ovary/ Ovule Culture

            Somaclonal Variations, Gametoclonal Variation

            Plant Tissue Culture and Genetic Transformation

Plant Growth Regulators

            Plant Growth Regulators Used in Plant Tissue Culture, Hormones,

            Full Hormones, Auxin, Cytokinin, Cytokinins, Cytokinins2, GA, Ethylene signaling, Bioassay for Gibberellin,

            Auxin, Gibberllin, Abscisic Acid, Ethylene Gas, Ethylene signaling

            Plant Growth Regulators Used in Plant Tissue Culture, Plant Growth Regulators, somatic embryogenesis

Protoplasts - Methods of Isolation, Culture and Regeneration of Protoplasts,

Protoplast Fusion and Somatic Hybridization

Transgenic Plants

            Agrobacterium, Agrobacterium2, Gene transfer, Plant Transformation

            Methods for production of transgenic plants / Gene delivery methods,

Plant vectors- Gene Manipulation with Agrobacterium Tumaefaciens,

Binary vectors, cointegration pTi and Ti plasmid

Viral gene transfer- Bromo mosaic virus (BMV), CaMV, Gemini virus, TMV

Direct Gene Transfer methods - microinjection, macroinjection

Direct DNA uptake, Lipofection, Electroporation, Particle gun method, Biolistic

by CaCl₂ and PEG

            Examples of Transgenic Plants, Transgenic Plants Applications, Adventitious regeneration

Herbicide resistance, pest resistant, drought resistant, salt resistant

Nitrogen fixation and biofertilizer, Nitrogen fixatation gene

Secondary Metabolite Production Alternative for Secondary Etabolite Production

Production of Secondary Metabolites

Biochemical Products of Interest - PART I- Pharmaceuticals

Biochemical Products of Interest - Part II- Other Products

Germplasm Preservation: Cryobiology

Biological control of pests and disease of crop plants, biopesticides

Dimensions of Plant Tissue Culture Research

Designing a Plant Micro propagation Laboratory

Plant Time Scale

Plant Tissue Culture Terminology