Biophysics / Instrumentation notes Light Microscopy, Optics of Microscopy, Optical Systems, Refractive Index, Numerical Aperture, Resolution, Magnification Range, Polarized Light Microscopy, Phase contrast, Dark field Microscopy, Fluorescence, Confocal Microscopy, 2, Imaging Modes of Confocal Microscopy, Differential Interference Contrast, Types Stereomicroscopy, Fluorescence Microscopy, Atomic Force Microscopy Centrifugation Principle, types, application, Theory Chromatography Principle, types, application, Diagram and electrophoretogram
Ion exchange, affinity chromatography, thin layer, gas chromatography,
High pressure liquid (HPLC) chromatography, gel exclusion, hydrophobic (reverse phase),
Electrophoresis Principle, types, application
Spectroscopy Principle, types, application, Overview Electromagnetic radiation and equation for wave; Quantization of energy and its calculation; Definition and importance of spectroscopy; Region of diff spectra; Absorption and emission spectra; Instruments; Signal-to-noise ratio; Spectral width; Signal intensity; Fourier Transformation, Emmission Spectroscopy, Absorption Spectroscopy, Mass Spectrometry, UV-VIS Spectroscopy, Theory of electronic spectra (atomic and band spectra, L-B law, application and exception); Instrumentation; Chromophore, auxochrome; Woodward�s rule; Solvent effect (Bathochromic shift etc); Application to small biomolecules.
IR Spectroscopy, Vibrational Spectroscopy, Vibration (different modes, di- and poly atomic); Frequency, wavenumber; Hook�s law; Instrumentation; Vibration-rotation spectrum of CO2; Factors influencing vibrational frequency (vibronic coupling, H-bond, electronic factors, bond angles etc). Raman Spectroscopy, Introduction to Raman; Pure rotational and vibrational Raman spectra; mutual exclusion principle; Application to simple and biomolecules.
Radiation dosimetry Radioactive isotopes and half life, Autoradiography,
Effect of radiation on biological system,
Principle; Chemical shift (different unit) and factors influencing chemical shift; Correlation data; Solvent effect; Spin-spin coupling and splitting and factors involve; Relaxation (1D spectra); Application to small and biomolecules.
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