Molecular Cloning: A Laboratory Manual by Joseph Sambrook, David W. Russell

Free eBook PDF Molecular Cloning: A Laboratory Manual by Joseph Sambrook, David W. Russell Download
Chapter 1. Plasmids and Their Usefulness in Molecular Cloning
Chapter 2. Bacteriophage lambda and Its Vectors
Chapter 3. Working with Bacteriophage M13 Vectors
Chapter 4. Working with High-capacity Vectors
Chapter 5. Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis
Chapter 6. Preparation and Analysis of Eukaryotic Genomic DNA
Chapter 7. Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells
Chapter 8. In vitro Amplification of DNA by the Polymerase Chain Reaction
Chapter 9. Preparation of Radiolabeled DNA and RNA Probes
Chapter 10. Working with Synthetic Oligonucleotide Probes
Chapter 11. Preparation of cDNA Libraries and Gene Identification
Chapter 12. DNA Sequencing
Chapter 13. Mutagenesis
Chapter 14. Screening Expression Libraries
Chapter 15. Expression of Cloned Genes in Escherichia coli
Chapter 16. Introducing Cloned Genes into Cultured Mammalian Cells
Chapter 17. Analysis of Gene Expression in Cultured Mammalian Cells
Chapter 18. Protein Interaction Technologies


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