Culture of Human Stem Cells

Culture of Human Stem Cells
Wiley-Liss | 0470052465 | Edition - June 22, 2007 | PDF | 343 pages |10 MB| Rapidshare&Megaupload

This book collects and focuses on commonly used and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells. It presents clear, concise information that is immediately useful and applicable. Each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. Each chapter includes a comprehensive list of suppliers for all equipment used in the protocols presented, with complete contact information for the suppliers in a special appendix at the end of the book. Culture of Human Stem Cells also includes three general chapters on quality control, legal and ethical issues, and cryopreservation.

This concise, one-stop resource is an invaluable introduction to the field of stem cell biology and culture for all researchers and clinical scientists with interests in tissue replacement therapies. It is also an essential textbook for teachers and students who are involved with this fascinating and crucial area of study and the therapeutic potential of stem cell research.

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Fundamentals of Tissue Engineering and Regenerative Medicine

Ulrich Meyer, Thomas Meyer, Jorg Handschel, Hans Peter Wiesmann, "Fundamentals of Tissue Engineering and Regenerative Medicine"
Springer | 2009-04 | ISBN: 3540777547 | 1076 pages | PDF | 22,7 MB

"Fundamentals of Tissue Engineering and Regenerative Medicine" provides a complete overview of the state of the art in tissue engineering and regenerative medicine. Tissue engineering has grown tremendously during the past decade. Advances in genetic medicine and stem cell technology have significantly improved the potential to influence cell and tissue performance, and have recently expanded the field towards regenerative medicine. In recent years a number of approaches have been used routinely in daily clinical practice, others have been introduced in clinical studies, and multitudes are in the preclinical testing phase. Because of these developments, there is a need to provide comprehensive and detailed information for researchers and clinicians on this rapidly expanding field.

This book offers, in a single volume, the prerequisites of a comprehensive understanding of tissue engineering and regenerative medicine. The book is conceptualized according to a didactic approach (general aspects: social, economic, and ethical considerations; basic biological aspects of regenerative medicine: stem cell medicine, biomolecules, genetic engineering; classic methods of tissue engineering: cell, tissue, organ culture; biotechnological issues: scaffolds; bioreactors, laboratory work; and an extended medical discipline oriented approach: review of clinical use in the various medical specialties). The content of the book, written in 68 chapters by the world’s leading research and clinical specialists in their discipline, represents therefore the recent intellect, experience, and state of this bio-medical field.

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Stem Cells & Regenerative Medicine: From Molecular Embryology to Tissue Engineering

Stem Cells & Regenerative Medicine: From Molecular Embryology to Tissue Engineering (Stem Cell Biology and Regenerative Medicine)
Humana Press; 1st Edition. edition (November 1, 2010) | ISBN: 1607618591 1607618605 | 632 pages | PDF | 11 MB

Defined as, “The science about the development of an embryo from the fertilization of the ovum to the fetus stage,” embryology has been a mainstay at universities throughout the world for many years. Throughout the last century, embryology became overshadowed by experimental-based genetics and cell biology, transforming the field into developmental biology, which replaced embryology in Biology departments in many universities. Major contributions in this young century in the fields of molecular biology, biochemistry and genomics were integrated with both embryology and developmental biology to provide an understanding of the molecular portrait of a “development cell.” That new integrated approach is known as stem-cell biology; it is an understanding of the embryology and development together at the molecular level using engineering, imaging and cell culture principles, and it is at the heart of this seminal book. Stem Cells and Regenerative Medicine: From Molecular Embryology to Tissue Engineering is completely devoted to the basic developmental, cellular and molecular biological aspects of stem cells as well as their clinical applications in tissue engineering and regenerative medicine. It focuses on the basic biology of embryonic and cancer cells plus their key involvement in self-renewal, muscle repair, epigenetic processes, and therapeutic applications. In addition, it covers other key relevant topics such as nuclear reprogramming induced pluripotency and stem cell culture techniques using novel biomaterials. A thorough introduction to stem-cell biology, this reference is aimed at graduate students, post-docs, and professors as well as executives and scientists in biotech and pharmaceutical companies.

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Medicines from Animal Cell Culture

Medicines from Animal Cell Culture
John Wiley & Sons | 0470850949 | Edition - 2007-10-05 | PDF | 672 pages | 6.9 MB | Rapidshare & Megaupload & Aliceinwire

Medicines from Animal Cell Culture focuses on the use of animal cell culture, which has been used to produce human and veterinary vaccines, interferon, monoclonal antibodies and genetically engineered products such as tPA and erythropoietin. It also addresses the recent dramatic expansion in cell-based therapies, including the use of live cells for tissue regeneration and the culture of stem cells.


Medicines from Animal Cell Culture:
* Provides comprehensive descriptions of methods for cell culture and nutrition as well as the technologies for the preservation and characterisation of both the cells and the derived products
* Describes the preparation of stem cells and others for use in cell-based therapies – an area of burgeoning research
* Includes experimental examples to indicate expected results
* Covers regulatory issues from the UK, the EU and the USA and reviews how these are developing around the world
* Addresses the key issues of standardisation and validation with chapters on GLP and GMP for cell culture processes

Delivering insight into the exciting world of biological medicines and directions for further investigation into specific topics, Medicines from Animal Cell Culture is an essential resource for researchers and technicians at all levels using cell culture within the pharmaceutical, biotechnology and biomedical industries. It is of value to laboratory managers in these industries and to all those interested in this topic alike.


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Principles Of Animal Cell Culture (students Compendium)

Principles Of Animal Cell Culture (students Compendium) By Dr. Basant K. Sinha & Dr. Rinesh Kumar
International Book Distributing Co. | English | 2008 | ISBN: 818189250X | 305 Pages | PDF | 13.3 MB

Animal cell culture is playing a pivotal role in the various modern researches. Though the foundation stone of animal cell culture was laid for the isolation and characterization of different viruses but the recent developments in the field of biotechnology and molecular biology have given various new dimensions to this technology. Cell culture has provided a new tool for the better understanding of diagnosis and molecular biology in many areas of biological systems including bacterial, viral and parasitic diseases. The different cell cultures are being used in virology for the last five decades and one of its application has been utilized successfully in various types of vaccine production. To quote virologist Payton Rous of late we have begun to study viruses not only for what they themselves mean, but for what they can reveal about the intrinsic cell structure, and functions through located injuries that they do". More recent discoveries such as production of various proteins and enzymes through recombinant DNA technology require the use of cell culture to study the exact gene expression, have fully utilized where the available evidences justify it and where their use is consistent with the effort involved and their routine application is recommended. With the advancement in tissue culture technology, scientists are now on the threshold of bringing sea changes in improving the life of human as well as livestock and thus it has become a source of unending fascination for different scientists.

The present work is directed primarily to the students of biotechnology, keeping in view the University Grants Commission Syllabus on animal cell culture prescribed for vocational course both for undergraduate and postgraduate students and also for the scientists in the medical, veterinary and biological sciences, whose curiosity and efforts are responding to the stimulus of current interest in biotechnology.

It is hoped that it may have some value as a source of providing more readily obtained information on different topics. It is expected that some deficiencies might have persisted in this book, it is anticipated that these deficiencies may be eliminated by diligent supplementing readings and by classroom lecture materials. Though this is the first endeavour, constructive criticism will be welcomed by the author to be included in the next edition.

Contents: 1. History of development of cell culture. 2. Natural surroundings of animal cell. 3. Metabolic capabilities of animal cell. 4. Simulating natural conditions for growing animal cells. 5. Serum and serum free media. 6. Cell culture system. 7. Secondary culture: transformed animal cell. 8. Commonly used cell lines and their uses. 9. Growth kinetics of cells in culture. 10. Gene expression and application of animal cell culture for its studies. 11. Organ culture. 12. Transfection. 13. Cell fusion. 14. Selectable marker and antibiotic resistance. 15. Hybridoma and monoclonal antibodies. 16. Transplantation of cultured cell. 17. Differentiation of cells. 18. Transgenesis and transgenic animals. 19. Apoptosis, necrosis, senescence and quiescence. 20. Embryo transfer technology. References. Index.

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Cell Biology: A Laboratory Handbook, Four-Volume Set

Cell Biology: A Laboratory Handbook, Four-Volume Set
Publisher: Academic Press | Pages: 2328 | 2005-11-16 | ISBN 0121647307 | PDF | 140 MB

This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for further ease of use. This collection enables researchers at all stages of their careers to embark on basic biological problems using a variety of technologies and model systems.

This thoroughly updated third edition contains 165 new articles in classical as well as rapidly emerging technologies.

Topics covered include:

* Cell and Tissue Culture: Associated Techniques, Viruses, Antibodies, Immunocytochemistry (Volume 1)
* Organelle and Cellular Structures, Assays (Volume 2)
* Imaging Techniques, Electron Microscopy, Scanning Probe and Scanning Electron Microscopy, Microdissection, Tissue Arrays, Cytogenetics and In Situ Hybridization, Genomics and Transgenic Knockouts and Knock-down Methods (Volume 3)
* Transfer of Macromolecules, Expression Systems, Gene Expression Profiling (Volume 4)
* Indispensable bench companion for every life science laboratory
* Provides the latest information on the plethora of technologies needed to tackle complex biological problems
* Includes numerous illustrations, some in full color, supporting steps and results


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Cell and Tissue Culture: Laboratory Procedures

Cell and Tissue Culture: Laboratory Procedures
Wiley | December 16, 1998 | ISBN-10: 0471982555 | 352 pages | PDF | 39.56 mb

Cell and Tissue Culture: Laboratory Procedures in Biotechnology Edited by Alan Doyle Centre for Applied Microbiology & Research, Porton Down, Salisbury, UK. and J. Bryan Griffiths Scientific Consultancy & Publishing, Porton, Salisbury, UK. Cell and Tissue Culture: Laboratory Procedures in Biotechnology introduces the reader to animal cell culture methods describing the key cells, core techniques, how to scale up the culture for commercial production, and regulatory aspects. This book provides easy to follow, step-by-step protocols, with trouble-shooting tips and notes on time considerations. Alternative procedures, background information and references supplement the main procedures described. Other features include:

* Experimental examples to indicate expected results;
* Quick reference symbols such as safety icons with warning notes; and,
* A list of suppliers is provided to allow easy access to laboratory products.

Written by a team of international scientists, Cell and Tissue Culture: Laboratory Procedures in Biotechnology will be of interest to researchers, technicians and process engineers using cell culture within the biotechnology, biomedicine and pharmaceutical industrie


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Perfect Written English: All You Need to Get It Right First Time

 

 

Chris West, "Perfect Written English: All You Need to Get It Right First Time "
Random House UK | 2008 | ISBN: 1847945031 | 176 pages | PDF | 1,3 MB

This is an indispensable guide to mastering grammar and improving your writing style. Covering every aspect of good writing—from the correct way to use commas to the most persuasive structure for an argument—it gives step-by-step guidance on how to write fluently and convincingly. With helpful tips on avoiding common mistakes and practical sections on writing everything from personal webpages to corporate sales reports, this resource has all you need to make sure you get your message across elegantly and effectively.

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Chemical Communication in Crustaceans

Thomas Breithaupt, "Chemical Communication in Crustaceans"
Publisher: S///r | 2010 | ISBN: 038777100X | PDF | 565 pages | 9.6 MB

The crustaceans are ecologically and economically important organisms. They constitute one of the dominant invertebrate groups on earth, particularly within the aquatic realm. Crustaceans include some of the preferred scientific model organism, profitable aquaculture specimen, but also invasive nuisance species threatening native animal communities throughout the world. Chemoreception is the most important sensory modality of crustaceans, acquiring important information about their environment and picking up the chemical signals that mediate communication with conspecifics.

Significant advances have been made in our understanding of crustacean chemical communication during the past decade. This includes knowledge about the identity, production, transfer, reception and behavioral function of chemical signals in selected crustacean groups. While it is well known that chemical communication is an integral part of the behavioral ecology of most living organisms, the intricate ways in which organisms allocate chemicals in communication remains enigmatic. How does the environment influence the evolution of chemical communication? What are the environmental cues that induce production or release of chemicals? How do individuals economize production and utilization of chemicals? What is the importance of molecule specificity or mix of a molecule cocktail in chemical communication? What is the role of chemical cues in multimodal communication? How does the ontogenetic stage, the sex or the physiological status of an individual affect its reaction to chemical cues? Many of these questions still represent important challenges to biologists.


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